The StemAb RNA Transfection Kit is a proprietary mixture of lipid components is specifically designed for in vitro RNA transfection. This kit has been shown to deliver mRNA, siRNA and miRNA. StemAb RNA Transfection Kit provides greater than or equal to 90% transfection efficiency of mRNA into a range of cell types with greater than 95% viability; these cell types include human embryonic stem (hES) cells, Jurkat cells, human fibroblasts, and HEK293T-cells. StemAb RNA Transfection Kit has been successfully used to deliver RNA to human ES cells, dendritic cells, and retinal pigment epithelial cells. There is no need to serum-starve cells using this kit as the transfection efficiency remains greater than or equal to 90% in the presence of serum.
Higher levels of protein expression with StemFect RNA Transfection Kit. BJ fibroblasts were transfected with GFP mRNA. GFP expression was assessed at 18h post-transfection.
Titrate protein expression by modifying the amount mRNA used for transfection. BJ fibroblasts were transfected with eGFP mRNA. Transfection efficiency and GFP intensity were determined at 18h post-transfection.
High efficiency delivery of siRNA to hES cells. HUES9 cells were transfected with siRNA using StemAb RNA Transfection Kit. (A) Greater than 90% of hES cells were transfected with FAM labeled control siRNA, 75nM (250ng).
High efficiency delivery of siRNA to hES cells. HUES9 cells were transfected with siRNA using StemAb RNA Transfection Kit. (C) Transfection with 10nM and 20nM Oct4 siRNA results in altered colony morphology.
High efficiency delivery of siRNA to hES cells. HUES9 cells were transfected with siRNA using StemAb RNA Transfection Kit. (B) Transfection with Oct4 siRNA, 20nM (250ng), resulted in altered colony morphology and expression of SSEA-1 indicating differentiation.
Human embryonic stem (hES) cells remain undifferentiated after transfection. H1 hES cells cultured in NutriStem were transfected with eGFP mRNA using StemAb RNA Transfection Kit. (A) Greater than 95% of cells maintained pluripotency marker expression, SSEA-4, 24 hours post-transfection.
Human embryonic stem (hES) cells remain undifferentiated after transfection. H1 hES cells cultured in NutriStem were transfected with eGFP mRNA using StemAb RNA Transfection Kit. (B) Normal colony morphology is maintained 20 hours post-transfection.
reprocell的StemAb碱性磷酸酶染色试剂盒II（1个试剂盒（50个测定））
碱性磷酸酶（AP）在多能干细胞中高水平表达。AP在碱性条件下使核苷酸，蛋白质和生物碱去磷酸化。染色后，未分化的细胞显示为红色或紫色，而分化的细胞显示为无色。
胚胎干细胞（ES）和诱导性多能干细胞（iPS）的未分化状态可以通过碱性磷酸酶（AP）的高水平表达来表征，该表达与其他表面标志物的表达一起表明未分化的细胞具有自我分化的潜力-更新。AP是一种水解酶，负责在碱性条件下对诸如核苷酸，蛋白质和生物碱之类的分子进行去磷酸化。当使用AP染色试剂盒对固定的ES或iPS细胞进行染色时，未分化的细胞显示为红色或紫色，而分化的细胞则显示为无色。
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