Third Generation RNA Kit for Cellular Reprogramming of Fibroblasts, Blood, and Urine
The latest evolution in Stemgent RNA reprogramming, the StemRNA 3rd Gen Reprogramming Kit (formerly called the StemRNA-NM Reprogramming Kit), combines non-modified RNA and microRNA technology to provide a kit for stem cell researchers with a new level of versatility, simplicity and time savings, enabling cellular reprogramming of human fibroblasts, and cells from blood and now urine for difficult to reprogram patient samples.
Key Benefits
- Flexible technology generates high-quality human iPS cell lines from multiple target cell typesOut-of-the-box reprogramming of cells from skin (fibroblasts), blood (endothelial progenitor cells; EPCs) and urine (urine-derived progenitor cells; UPCs)
- High efficiency, non-integrating reprogrammingStemRNA-3rd Gen requires a few as four additional reagents. Double stranded microRNAs enhance reprogramming efficiency, providing iPS cells with high efficiency (up to 4% from fibroblasts, up to 3% from EPCs, up to 0.5% from UPCs).
- Time-saving protocol delivers faster results facilitating higher throughputColonies ready to pick in 10-14 days from fibroblasts and 12-16 days from EPCs or UPCs. No screening needed
| StemRNA-3rd Gen Reprogramming Kit (00-0076) | |||
|---|---|---|---|
| Feature | Fibroblasts | Urine (UPCs) | Blood (EPCs) |
| No. wells per kit | 9 | 3 | 3 |
| No Transfections required | 4 | 6-8 | 6-8 |
| Days to primary iPS cell colonies | 10-14 | 12-14 | 12-14 |
| Reprogramming efficiency | 2-+4% | 0.1-0.5% | 0.4-3% |
| Screening Required | No | No | No |
| Xeno-compatible protocol | Yes | No | No |
| GMP-compatible RNA manufacturing protocol | Yes | Yes | Yes |
P7 primary iPS cells derived from UPCs were stained using the StainAlive TRA-1-60 Antibody (Cat. No. 09‑0068) and DAPI after expansion in NutriStem hPSC XF Medium (Cat. No. 01‑0005)
iPS cells derived from fibroblasts using the StemRNA-3rd Gen Reprogramming Kit were cultured on iMatrix-511 (Cat. No. NP892‑011) in NutriStem hPSC XF Medium(Cat. No. 01‑0005)for 7 passages. Magnification: 4x
Timeline for StemRNA 3rd reprogramming of fibroblasts
Download our StemRNA-3rd Gen Reprogramming Posters:
ISSCR 2015
ISSCR 2016
reprocell的StemAb碱性磷酸酶染色试剂盒II（1个试剂盒（50个测定））
碱性磷酸酶（AP）在多能干细胞中高水平表达。AP在碱性条件下使核苷酸，蛋白质和生物碱去磷酸化。染色后，未分化的细胞显示为红色或紫色，而分化的细胞显示为无色。
胚胎干细胞（ES）和诱导性多能干细胞（iPS）的未分化状态可以通过碱性磷酸酶（AP）的高水平表达来表征，该表达与其他表面标志物的表达一起表明未分化的细胞具有自我分化的潜力-更新。AP是一种水解酶，负责在碱性条件下对诸如核苷酸，蛋白质和生物碱之类的分子进行去磷酸化。当使用AP染色试剂盒对固定的ES或iPS细胞进行染色时，未分化的细胞显示为红色或紫色，而分化的细胞则显示为无色。
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