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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
reprocell/Stemolecule ec23/Synthetic retinoid ec23 is also a potent inducer of neurogenesis by adult neuroprogenitor cells. Rat adult hippocampal progenitor cells (AHPCs) exposed to either 1 uM ec23 or ATRA differentiate into neural cell types. Differenti
产品编号:SRP002-base
市  场 价:¥1360.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$68.00
品      牌: reprocell
公      司:reprocell
公司分类:
reprocell/Stemolecule ec23/Synthetic retinoid ec23 is also a potent inducer of neurogenesis by adult neuroprogenitor cells. Rat adult hippocampal progenitor cells (AHPCs) exposed to either 1 uM ec23 or ATRA differentiate into neural cell types. Differenti
商品介绍

ec23 is a pan-RAR receptor agonist that is stable and maintains the same biological activity as ATRA (all trans retinoic acid). ATRA is well known for its ability to regulate cell differentiation. However, retinoids such as ATRA are excellent chromophores and efficiently absorb light and isomerise. This is not a desirable property for cell culture reagents where consistency and reliability of a molecule is important for robust, reproducible results.

REPROCELL routinely test the stability of the our synthetic compounds in line with their development as stable alternatives to known biological molecules. The structural stability of ec23 and ATRA were examined by NMR during prolonged exposure to light. ATRA shows significant isomerisation and degradation whereas ec23 remains completely intact.

Robust induction of neurogenesis by ec23

The synthetic retinoid, ec23 is a potent inducer of pluripotent stem cell differentiation. Human TERA2.cl.SP12 pluripotent stem cells exposed to either 10 uM ec23 or ATRA form populations of terminally differentiated neurons. Immunocytochemical staining for neurofilament-200 was performed on differentiated cultures and the number of positive cells quantified. Results show that ec23 is a more potent inducer of neurogenesis than ATRA. In direct comparison to ATRA, synthetic retinoid ec23 induces larger numbers of neural cells with less variability and with the added advantage of compound stability.

Synthetic retinoid ec23 is also a potent inducer of neurogenesis by adult neuroprogenitor cells. Rat adult hippocampal progenitor cells (AHPCs) exposed to either 1 uM ec23 or ATRA differentiate into neural cell types. Differentiation induced by exposure to ec23 was consistent and reproducible.

Human TERA2.cl.SP12 EC cells exposed to either ec23 or ATRA form populations of terminally differentiated neurons after 21 days. Immunocytochemical staining for neurofilament-200 was performed on differentiated cultures.

品牌介绍

reprocell的StemAb碱性磷酸酶染色试剂盒II(1个试剂盒(50个测定))

碱性磷酸酶(AP)在多能干细胞中高水平表达。AP在碱性条件下使核苷酸,蛋白质和生物碱去磷酸化。染色后,未分化的细胞显示为红色或紫色,而分化的细胞显示为无色。


胚胎干细胞(ES)和诱导性多能干细胞(iPS)的未分化状态可以通过碱性磷酸酶(AP)的高水平表达来表征,该表达与其他表面标志物的表达一起表明未分化的细胞具有自我分化的潜力-更新。AP是一种水解酶,负责在碱性条件下对诸如核苷酸,蛋白质和生物碱之类的分子进行去磷酸化。当使用AP染色试剂盒对固定的ES或iPS细胞进行染色时,未分化的细胞显示为红色或紫色,而分化的细胞则显示为无色。


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